Biotin – Gastrin Releasing Peptide, human

Biotin-Gastrin Releasing Peptide, Human (Biotin-GRP) is a chemically modified, bioactive peptide where the human Gastrin-Releasing Peptide (GRP) is covalently conjugated to a biotin molecule, typically via a linker at the N-terminus or a lysine residue. This conjugation preserves the native biological activity of GRP while introducing a versatile biotin tag that enables high-affinity binding to streptavidin or avidi…

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	Biotin-Gastrin Releasing Peptide, Human (Biotin-hGRP)
CAS NO.	133062-24-7
Appearance	White to off-white lyophilized powder
Molecular Formula	C140H218N40O33S3
Molecular Weight	3085.74 g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water (≥ 5 mg/mL), dilute acetic acid, and DMSO; forms a clear solution at 1 mg/mL in water.
Endotoxin	< 1.0 EU/mg
Biological Activity	Retains native hGRP activity: stimulates gastrin release and acts as a mitogen Biotin label enables detection via streptavidin-based assays
Storage Conditions	Store at -20°C to -80°C in a desiccated environment Avoid repeated freeze-thaw cycles; aliquot for long-term storage
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

Biotin-GRP functions identically to native human GRP by specifically binding and activating the Gastrin-Releasing Peptide Receptor (GRPR or BB2 receptor), a G-protein coupled receptor of the Gq/11 subtype. Upon binding, it triggers the canonical GRP signaling cascade: activation of phospholipase C (PLC), generation of inositol trisphosphate (IP₃) and diacylglycerol (DAG), leading to intracellular calcium mobilization and protein kinase C (PKC) activation.

2.PRODUCT INDICATIONS

GRPR Detection & Localization: Fluorescent or enzymatic immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to visualize GRPR expression and distribution in cells, tissues, or tumor samples.
Affinity Pull-Down & Proteomics: Isolating GRP-bound receptors or associated protein complexes from cell lysates using streptavidin-coupled beads for mass spectrometry or Western blot analysis.
Biosensor & Assay Development: Immobilization on streptavidin-coated surfaces (e.g., SPR chips, ELISA plates) for binding kinetics studies, receptor-ligand interaction analysis, or high-throughput drug screening.
Receptor Trafficking Studies: Tracking the internalization and intracellular fate of the GRP-GRPR complex in real-time using fluorescent streptavidin conjugates.

3.CLINICAL EFFICACY OF PRODUCT

Research Utility: Its value lies in enabling critical preclinical research that informs clinical translation. It is extensively used to characterize GRPR expression profiles in human cancers (e.g., prostate, breast, lung), which is a prerequisite for developing targeted therapies and diagnostics.
Diagnostic Development: The biotin-streptavidin system is a cornerstone in vitrodiagnostic technology. Biotin-GRP can be utilized in the development and calibration of research-use-only (RUO) or prototype in vitrodiagnostic assays to detect GRPR levels in patient samples.
Therapeutic Insights: Research using this tool helps validate GRPR as a target, screen for antagonists, and understand signaling pathways, indirectly contributing to the development of GRPR-targeted radioligands and therapeutics currently in clinical trials.
No Therapeutic Use: This conjugate is not administered to humans. Clinical efficacy data pertains to the GRP/GRPR pathway itself, not to this detection reagent.

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.